|
1
|
p Psal 14
r r2 5
c supD 11
t t
p PBAD 14
r r2 5
c T7ptag 5
t t
p PT7 14
r r2 5
c GFP 3
t t
# Arcs
supD ind PT7
T7ptag ind PT7
NahR ind Psal
AraC ind PBAD
|
|
|
2
|
<t t
<c c2 4
<r r1 1
<p p1 6
p p2 2
r r2 5
c c1 6
t t
# Arcs
c1 rep p1
c2 rep p2
y rep c1-p1
x rep c2-p2
|
|
|
3
|
p p 13 nl
r r 13 nl
c tetR 13
t t 13 nl
p P_LtetO 2
> FRT_f 2
<p pBAD 6
r r 1 nl
c flp_e 2
d ssrA 12
t
< FRT_r 2
> loxP_f 6
<p P_A1lacO 4
r r 5 nl
c cre 6
d ssrA 12
t
< loxP_r 6
r r 3 nl
c gfp 4
t
# Arcs
tetR rep P_LtetO
aTc rep tetR-P_LtetO
Ara ind pBAD
IPTG ind P_A1lacO
|
|
|
4
|
o A 6
p pX
r r 1
c X 1
r r 9
c A 9
t
p pA 9
r r 2
c X' 2
t
o B 6
# Arcs
C rep pC
c3 rep p3
Zin ind pZ
Yin ind pY
Xin ind pX
B rep pB
A rep pA
|
|
|
5
|
o C 4
> X' 2
p pY
r r 4
c Y 4
r r 10
c B 10
t
p pB 10
r r 6
c Y' 6
t
> Y' 6
p pZ
r r 7
c C 7
r
c out-1
t
p pC 7
r
c out-0
t
> Y' 6
> Y 4
p pZ
r r 7
c C 7
r
c out-3
t
p pC 7
r
c out-2
t
> Y 4
> X' 2
o D 2
# Arcs
C rep pC
c3 rep p3
Zin ind pZ
Yin ind pY
Xin ind pX
B rep pB
A rep pA
|
|
|
6
|
p
p
r
c
t
|
|
|
7
|
P pb 8
P pa 8
r r9 4
c g9 6
T t
P pn_3 8
P pc 8
r r8 4
c g8 6
T t
P pn_5 8
P pd 8
r r7 4
c g7 6
T t
P pout_x 8
# Arcs
g7 rep pout_x
g8 ind pn_5
g9 ind pn_3
|
|
|
8
|
x XBaI 8
<t t
<c c2 2
<r r1 1
<p p1 6
x SpeI 8
p p2 2
r r2 5
c c1 6
t t
z Ori 4
# Arcs
c1 rep p1
c2 rep p2
y rep c1-p1
x rep c2-p2
|
|
|
9
|
rgbcolors
#00aa00
#aa0aa0
#aa0000
#aaaa00
#aa0000
#aaaa00
#aa0000
#aaaa00
#aa00aa
#aa00aa
#aa00aa
#aaaa00
#aaaa00
#aaaa00
o A 1
<t t
<c c2 2
<r r1 1
<p p1 6
p p2 2
r r2 5
c c1 6
t t
o B 1
v KanR 1
# Arcs
c1 rep p1
c2 rep p2
y rep c1-p1
x rep c2-p2
|
|
|
10
|
p p1
c c1 5
p p1
c c2 8
c c3 3
c c4 6
p p3
c c5 11
p p1
c c6 1
p p5
c c7 2
c c8 4
# Arcs
c1 ind p1
c6 ind p5
c7 rep p1
c5 rep p5
|
|
|
11
|
o A 2
<t t
<c c2 2
<r r1 1
<p p1 6
p p2 2
r r2 5
c c1 6
t t
o B 2
v KanR 1
# Arcs
c1 rep p1
c2 rep p2
y rep c1-p1
x rep c2-p2
|
|
|
12
|
p p 2
r r 2
c c 2
r r 3
g g 3
t t 2
# Arcs
c rep p
c rep r
|
|
|
13
|
p p 14 nl
r r 2 nl
f fp 14-2
t t 6 nl
# Arcs
|
|
|
14
|
5 EcoRI 2
? 10bp_spacer 3
p PspaR 2
r RBS-spaRK 2
? post-RBS_spacer 2
c spaRK 2
t rrnB-T1 6
t rrnO 6
? post-rrnO_6bp_spacer 5
p PspaS 8
r RBS-spaS 8
? post-RBS_spacer 8
> Met 4
5 NheI 2
v pGFP-rrnB 3
# Arcs
spaRK ind PspaS
|
|
|
15
|
<c ccdA 2
<p pLux
p pTET
c LuxR 8
c lasI 8
# Arcs
|
|
|
16
|
p pRep 2
r rbs 1
c Rep1 3
c RFP 4
t term 1
p pRep2
r rbs 1
c GFP 5
t term 1
# Arcs
Rep1 rep pRep2
sm rep pRep
|
|
|
17
|
p J23104 1
r BCD2 9
c araC 5
t B0015 14
p I13453 2
r BCD2 9
c XFP 7
t B0015 14
|
|
|
18
|
p [I1] 8
p p 8
r r 4
c cds 6
t t
p p2 8
# Arcs
cds rep p2
|
|
|
19
|
> >R 3
p p3 8
p p 8
r r 4
c cds2 6
t t
p p2 8
r r 4
c cds1 6
< <R 3
t t
p p1 8
# Arcs
cds1 rep p1
cds2 rep p2
y rep c1-p1
x rep c2-p2
|
|
|
20
|
p plac 3
> RBS 4
c GNA1 9
> RBS 4
c PCM1 8
> RBS 4
c UAPM1 5
> RBS 4
c CHS3 12
<t stop 6
# Arcs
|
|
|
21
|
p p1 14 nl
r r 14 nl
c c1 8 nl
t t 14 nl
p p1 14 nl
r r 14 nl
c c 6 nl
t t 14 nl
p p2 8 nl
r r 14 nl
c c 4 nl
t t 14 nl
# Arcs
c1 rep p2
x rep c1-p2
|
|
